pe anti mouse foxp3  (Elabscience Biotechnology)

Journal: Cells

Article Title: Cancer Immunomodulatory Effect of Bidens pilosa L. in Mice: Suppression of Tumor-Associated Macrophages and Regulatory T Cells

doi: 10.3390/cells15020126

Figure Lengend Snippet: Effect of BPA and compounds 1 and 3 on the differentiation of macrophages and CD4 + T cells. ( A ) Representative flow cytometry plots illustrating the effect of BPA , 1 , and 3 on the differentiation of M2 macrophages. ( B ) Quantitative analysis of the ratios of M2 to M1 cells following treatment with various concentrations of BPA , 1 and 3 . ( C ) Representative flow cytometry plots illustrating the effect of BPA , 1 , and 3 on the differentiation of Tregs. ( D ) Histograms displaying the quantified results of the CD25 + Foxp3 + double-positive cell population following treatment with different concentrations of BPA , 1 and 3 . Statistical significance for all experiments is denoted as follows ( n = 3): compared to the M0 cells or Naïve CD4 + T cells, ## p < 0.01, ### p < 0.001, #### p < 0.0001; compared to M2-TAMs or Tregs, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. RFI means Relative Fluorescence Intensity.

Article Snippet: After incubated with PE anti-mouse Foxp3 antibody (Elabscience, Cat. E-AB-F1238D) at 4 °C for 50 min, cells were analyzed by flow cytometry for CD25 + Foxp3 + cell populations.

Techniques: Flow Cytometry, Fluorescence

Journal: Cells

Article Title: Cancer Immunomodulatory Effect of Bidens pilosa L. in Mice: Suppression of Tumor-Associated Macrophages and Regulatory T Cells

doi: 10.3390/cells15020126

Figure Lengend Snippet: Expression levels of mRNAs and proteins associated with TAMs and Tregs in BPA -treated mouse tumors. ( A ) Quantification of mRNA by qRT-PCR for TAM/Treg-associated tumor-promoting factors (Arg-1, YM-1, CCL2, CCL22, Foxp3, CD25, TGF-β, CXCR3, CCR4, CCR8, CCR10) and tumor-inhibitory factors (TNF-α, IL-1β, iNOS, CXCL10, CCR7). Data were normalized to 18 S rRNA and are shown relative to the model group (baseline control). ( B ) Representative immunoblots of tumor tissue for TAM- and Treg-associated proteins. Pro-tumor: CD206, Foxp3, CD25, PD-1, PD-L1; Anti-tumor: CD80, CD4, GITR. ( C ) Quantification of protein expression from Western blots. Values were normalized to α-tubulin, β-actin, or GAPDH as indicated. Statistical significance is denoted as follows ( n = 6): compared to the model group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After incubated with PE anti-mouse Foxp3 antibody (Elabscience, Cat. E-AB-F1238D) at 4 °C for 50 min, cells were analyzed by flow cytometry for CD25 + Foxp3 + cell populations.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot